FastQCFastQC Report
Wed 24 Apr 2019
CCRMBACXX_l03_n02_A2S7.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCCRMBACXX_l03_n02_A2S7.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16965132
Sequences flagged as poor quality0
Sequence length51
%GC54

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTAGAAC20750.012.25141411
CTCGAAT16850.011.3560361
CGCGATA7200.010.94178529
GTACTCG29200.010.0978054
GGAAAAT63150.09.69623851
TACTCGA30250.09.5983765
GGTAGAA29000.09.07664310
CGATAGT7500.09.00275531
AGTACTC34650.08.96497253
TATAAGG19950.08.6872275
CACTCGT26300.08.55310935
TACCCTA11850.08.5437439
TAGAACA30000.08.39900412
AATCGAA34850.08.3960295
CGTGACT16200.08.337092
AAAATCC50500.08.33534053
CTATAAG18700.08.3051214
TCAGGTA32550.08.2252917
GAATCGA29700.07.8058344
CGGCTAT24600.07.77842241
Produced by FastQC (version 0.11.5)