FastQCFastQC Report
Wed 24 Apr 2019
CCRMBACXX_l03_n02_A2S6.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCCRMBACXX_l03_n02_A2S6.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20389729
Sequences flagged as poor quality0
Sequence length51
%GC53

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTAGAAC31450.011.30219411
CGATAGT14000.09.002931
CTCGAAT22700.08.9255951
GGTAAGT19200.08.78794817
TCAGGTA43100.08.7696627
CGCGATA14300.08.65720929
TAGAACA42000.08.57040312
GGTAGAA41850.08.54744910
GCGAAAT38200.08.368461
CGAAATT31900.08.2562882
ACATGCG28300.08.1884896
GGAAAAT86550.08.1673871
AAAAATC71500.08.0283032
CAGGTAG44600.07.9198848
ACACGCG21150.07.7654296
AAAATCC70350.07.74348833
AATCCAG51100.07.6641435
TACCCTA20700.07.49949039
CGTAACG23950.07.419532342
CGTTAAC23850.07.357340321
Produced by FastQC (version 0.11.5)