FastQCFastQC Report
Wed 24 Apr 2019
CCRMBACXX_l03_n02_A2S5.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCCRMBACXX_l03_n02_A2S5.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences26648641
Sequences flagged as poor quality0
Sequence length51
%GC55

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTGACT27000.013.6729462
GTACTCG64650.012.0809574
TACTCGA64950.011.9556465
AGTACTC69000.011.7444353
GTAGAAC35850.011.42110511
GCGTGAC34500.011.2233681
GTGACTC36650.010.2570213
CTCGAAT25700.09.8106711
TCAGGTA46500.09.580087
AATCGAA57800.09.5794455
CGGCTAT41050.09.48739051
CACTCGT56950.09.44005835
CTCTATA57300.09.42159945
GGAAAAT102850.09.1273681
GCGGAAA68600.09.0901171
AAAATCC85350.09.0726393
TCACTCG59400.08.86216534
GAATCGA45500.08.8053984
ACTCGTG62650.08.79671236
TTACGAC23700.08.73759929
Produced by FastQC (version 0.11.5)