FastQCFastQC Report
Wed 24 Apr 2019
CCRMBACXX_l03_n02_A2S4.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCCRMBACXX_l03_n02_A2S4.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences23164110
Sequences flagged as poor quality0
Sequence length51
%GC55

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTGACT22050.011.6378172
GTAGAAC26800.011.41634211
TACTCGA56950.010.1965965
AGTACTC62300.010.04447653
GTACTCG57900.09.9516774
GCGTGAC29000.09.3926461
CTCGAAT22650.09.3424381
GCGGAAA49700.09.1494621
AAAATCC73200.08.5795333
CTATAAG25250.08.5574574
GAATTGA55200.08.4404614
AAAAATC69950.08.431192
GGAAAAT95950.08.3757521
CACTCGT44200.08.34640435
CTCTATA45950.08.32222345
GAATCGA40150.08.18468954
TATAAGG26500.08.0687645
GGTAGAA41150.07.87280610
TAGAACA38500.07.83016112
GTGACTC32800.07.82350833
Produced by FastQC (version 0.11.5)