FastQCFastQC Report
Wed 24 Apr 2019
CCRMBACXX_l03_n02_A2S3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCCRMBACXX_l03_n02_A2S3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences23881124
Sequences flagged as poor quality0
Sequence length51
%GC55

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACTCGA68650.011.6719395
GTACTCG69150.011.5227094
AGTACTC70750.011.4856893
GTAGAAC26550.011.35440711
CGTGACT20900.010.44728852
GCGTGAC27650.09.4443471
AAAATCC75300.09.2072943
GGAAAAT99500.08.9594461
ACTCGAG94400.08.8661536
CTCGAAT24500.08.4533881
ACGGTAC15050.08.37086420
CACGTAA28800.08.2816
GAATTGA54150.08.2717644
TATAAGG29400.08.1916235
CTCTATA39850.08.12835945
AATCGAA51000.08.120475
GAATCGA41150.08.09534554
CACTCGT40050.08.087954535
TAGAACA38350.08.03674712
CTATAAG28300.08.0330134
Produced by FastQC (version 0.11.5)