FastQCFastQC Report
Wed 24 Apr 2019
CCRMBACXX_l03_n02_A2S2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCCRMBACXX_l03_n02_A2S2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences26895663
Sequences flagged as poor quality0
Sequence length51
%GC54

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACTCG65100.012.0320244
TACTCGA68400.011.5499945
AGTACTC75700.010.7940473
GTAGAAC35800.010.74575611
CGTGACT21500.08.7946472
CACTCGT48750.08.72099135
CGCGATA10400.08.65710629
CTCTATA47350.08.64616745
GCGGAAA53850.08.6117041
GGAAAAT112350.08.5558341
AATTGAC45900.08.4832875
TAGAACA45450.08.46426712
AAAATCC85600.08.4149163
TCAGGTA51750.08.2603687
TCACTCG50400.08.25749134
CGGCTAT33100.08.0933161
GAATTGA61900.08.0360394
ACTCGAG105800.08.0169856
ACTCGTG52750.07.974440636
AATCGAA56750.07.73391685
Produced by FastQC (version 0.11.5)