FastQCFastQC Report
Wed 24 Apr 2019
CCRMBACXX_l03_n02_A2S1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCCRMBACXX_l03_n02_A2S1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19859729
Sequences flagged as poor quality0
Sequence length51
%GC54

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACTCG46700.011.0852974
CGTGACT19350.011.0515662
GTAGAAC21900.010.58079411
TACTCGA48900.010.3562985
AGTACTC52750.010.2414713
GGAAAAT85400.09.8324491
AAAATCC64950.09.1148623
ACACGCG22800.08.8809876
GCGTGAC25250.08.7372641
CGGCTAT26800.08.0639381
AAATCCT65100.08.0558484
AATCCTT54350.07.95119765
CTCGAAT20600.07.8682121
GGTAGGC19250.07.83054837
ATCTAGC24700.07.65131246
GGTAGAA32600.07.59113710
GAATTGA47450.07.5896214
GAATCGA35250.07.5345834
GTGACTC29600.07.52864843
GAAAATC72250.07.50862462
Produced by FastQC (version 0.11.5)