FastQCFastQC Report
Wed 24 Apr 2019
CCRMBACXX_l03_n01_undetermined.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCCRMBACXX_l03_n01_undetermined.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17746396
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGTCTG8400.031.4262315
CGTCTGA8350.031.07857116
CACGTCT9900.026.43766214
CACACGT10400.025.58852612
ACACGTC11850.022.45775813
AGACCGA7000.021.66001132
AGCACAC13000.021.15891810
CCGAGAC7600.020.76767729
GAGACCG7800.019.99751331
ACCGATC7300.019.5540234
CAGTCAC9250.019.24104927
GCACACG15250.017.89046511
ATCGGAA19650.017.6551132
AGCGGTT9700.017.52159111
TCGGAAG19850.017.4687563
CCAGTCA10850.016.81540926
GGAATGC10000.016.60069323
CGGAAGA22050.016.1316684
GATCGGA21500.016.0061781
CTCCAGT14950.015.76416724
Produced by FastQC (version 0.11.5)