FastQCFastQC Report
Wed 24 Apr 2019
CCRMBACXX_l03_n01_A2S9.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCCRMBACXX_l03_n01_A2S9.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18788117
Sequences flagged as poor quality0
Sequence length51
%GC52

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCAGATCTCGTATGCC333890.17771339192746138TruSeq Adapter, Index 9 (100% over 51bp)
CAAAAACAAAAGCAGAGCACGGCCGGTAAAATCAAAAGACGATGAACAAAC204160.10866442869181621No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGTCACG58100.031.39099328
CTCGTAT53850.030.6086642
TCGTATG55900.029.769443
ACGTCTG62800.029.72205215
CGTCTGA65250.028.53712516
CGTATGC59200.027.80654544
GTCACGA68000.026.6554829
CACGTCT75250.025.07354714
TCGGAAG76600.024.700363
ATCGGAA77500.024.3852672
CACACGT78400.024.2095512
CAGTCAC83850.022.42144227
TCACGAT82450.022.2566530
ACACGTC86550.022.0597713
GTATGCC79200.021.38178645
ACGATCA88400.020.6325132
TCTCGTA83250.020.17751741
CGGAAGA97050.019.9360454
TCAGATC92700.019.48587236
CCAGTCA94250.019.42242226
Produced by FastQC (version 0.11.5)