FastQCFastQC Report
Wed 24 Apr 2019
CCRMBACXX_l03_n01_A2S8.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCCRMBACXX_l03_n01_A2S8.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20092558
Sequences flagged as poor quality0
Sequence length51
%GC51

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT218620.10880645460871632No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGTAT41550.023.52080242
ACGTCTG67250.022.67229715
CGTCTGA70100.021.9109316
TCGTATG41300.021.7013143
CGTATGC42450.020.2119344
GTCACAC75700.019.66619129
TCGGAAG80700.019.2353533
ATCGGAA82900.018.5632132
CACACGT83700.018.45820212
CACGTCT84950.018.23954414
ACACGTC88850.017.43893413
AATCTCG69350.017.203239
AGTCACA86750.017.18708228
CAGTCAC90350.017.09962827
CACACTT94550.015.88857531
GATCGGA99800.015.554031
TCACACT98800.015.318530
TCTCGTA66250.015.22709141
GTCTGAA102200.015.22700217
TTGAATC98400.015.06516336
Produced by FastQC (version 0.11.5)