FastQCFastQC Report
Wed 24 Apr 2019
CCRMBACXX_l03_n01_A2S7.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCCRMBACXX_l03_n01_A2S7.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16965132
Sequences flagged as poor quality0
Sequence length51
%GC52

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGTAT33100.025.30415542
TCGTATG34950.023.70803343
ACGTCTG44550.023.57423815
CGTCTGA47050.022.22602716
CGTATGC39900.020.09022544
CACGTCT56450.018.92335514
TCGGAAG57550.018.3732573
ATCGGAA57000.018.3144842
CACACGT59500.018.06672512
CAGTCAC62550.017.11386727
ACACGTC67050.016.3006913
AGTCACC66650.016.09484328
CCAGTCA69200.014.88428226
GATCGGA71150.014.8527851
GTATGCC55100.014.79328645
TCTCGTA60500.014.21599541
CGGAAGA75350.014.212074
TCCAGTC75100.014.04434225
AGCACAC82600.013.5042410
TATAGGC35450.013.38550427
Produced by FastQC (version 0.11.5)