FastQCFastQC Report
Wed 24 Apr 2019
CCRMBACXX_l03_n01_A2S6.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCCRMBACXX_l03_n01_A2S6.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20389729
Sequences flagged as poor quality0
Sequence length51
%GC51

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT248960.1221006909900568No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATATCTCGTATGCC242160.11876567854334896TruSeq Adapter, Index 6 (100% over 51bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGTAT47100.027.87258542
AGTCACG54500.026.98593728
ACGTCTG56600.026.22308315
CGTCTGA59300.025.29457716
TCGTATG51150.025.2264243
TATCTCG58200.024.01925839
GTCACGC62400.023.74964329
CGTATGC54500.023.59409544
ATCGGAA68150.022.3501472
TCGGAAG69150.022.1879833
CACGTCT68650.021.94777714
CACACGT70950.021.29968312
CGCCAAT70350.020.7172533
ACGCCAA71300.020.50259432
ACACGTC75450.020.41679613
TCACGCC75100.019.85316830
CACGCCA74600.019.68545731
GATCGGA84250.018.3160511
CAGTCAC85750.018.30532827
GTATGCC71600.018.05354945
Produced by FastQC (version 0.11.5)