FastQCFastQC Report
Wed 24 Apr 2019
CCRMBACXX_l03_n01_A2S5.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCCRMBACXX_l03_n01_A2S5.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences26648641
Sequences flagged as poor quality0
Sequence length51
%GC53

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGTAT52850.024.4989642
TCGTATG53650.024.21868143
ACGTCTG59900.022.67613415
CGTCTGA59650.022.50726916
CGTATGC60950.021.5034644
GTCACAC67450.020.13788829
CACGTCT74100.018.6037814
TCGGAAG79400.017.6520843
ATCGGAA78600.017.632322
CACACGT80800.017.11680412
CACAGTG82150.016.427333
AGTCACA82950.016.26648928
CAGTCAC83450.016.2498727
AGTGATC82650.016.19395336
TCTCGTA85250.015.68948641
ACACGTC92150.015.30139813
GTATGCC93050.014.69032645
ACACAGT92600.014.62069732
CCAGTCA95550.014.23913626
CAGTGAT100150.013.67860535
Produced by FastQC (version 0.11.5)