FastQCFastQC Report
Wed 24 Apr 2019
CCRMBACXX_l03_n01_A2S4.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCCRMBACXX_l03_n01_A2S4.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences23164110
Sequences flagged as poor quality0
Sequence length51
%GC53

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGTAT39300.022.46073242
ACGTCTG51050.021.9818315
TCGTATG40100.021.95752743
CGTCTGA54800.020.68278916
AGTCACT58100.019.08226228
CGTATGC45600.018.86554144
ACTGACC62600.017.67579532
TCGGAAG68150.017.1989733
CACGTCT68050.016.85392814
ATCGGAA69400.016.760252
CACACGT70950.016.16504512
AATCTCG65400.015.66116939
ACACGTC78250.014.8294313
CAGTCAC80400.014.65663727
CACTGAC78000.014.30085831
GTCACTG79650.014.11702929
CGGAAGA94950.012.9368454
GACCAAT85750.012.93258535
GATCGGA91500.012.8897251
GTATGCC72650.012.4302445
Produced by FastQC (version 0.11.5)