FastQCFastQC Report
Wed 24 Apr 2019
CCRMBACXX_l03_n01_A2S3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCCRMBACXX_l03_n01_A2S3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences23881124
Sequences flagged as poor quality0
Sequence length51
%GC53

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAGGCATCTCGTATGCC263980.11053918567652009TruSeq Adapter, Index 3 (100% over 51bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGTAT49850.026.5588242
ACTTAGG57850.025.96918932
TCGTATG50600.025.81041743
ACGTCTG65000.023.76882615
CGTCTGA67000.022.95861416
TTAGGCA66100.022.902134
CGTATGC57600.022.4791244
TCACTTA68750.021.91626230
CACTTAG72050.020.94415731
AGTCACT72950.020.77777128
GTCACTT75900.019.97020329
CTTAGGC76900.019.8009433
TCGGAAG84200.018.9707013
CACGTCT83350.018.88673214
ATCGGAA85050.018.517672
CACACGT86450.018.15744612
ACACGTC96600.016.57552113
TCTCGTA83550.016.46591841
CAGTCAC96100.016.4277527
TAGGCAT93700.016.27656235
Produced by FastQC (version 0.11.5)