FastQCFastQC Report
Wed 24 Apr 2019
CCRMBACXX_l03_n01_A2S2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCCRMBACXX_l03_n01_A2S2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences26895663
Sequences flagged as poor quality0
Sequence length51
%GC52

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATCTCGTATGCC419230.15587271449675733TruSeq Adapter, Index 2 (100% over 51bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGTAT72850.030.07468842
TCGTATG72900.029.46866843
ACGTCTG92350.027.34674815
CGTCTGA94650.026.634716
CGTATGC79600.026.42306544
TATCTCG90200.025.92902839
GATGTAT100300.024.46788635
CACCGAT102600.024.24275231
ACCGATG104150.023.99086432
CACGTCT109000.023.31389214
TCGGAAG112200.022.7980023
ATCGGAA112200.022.6990152
CACACGT115100.022.21508412
GTATCTC118400.020.86908538
TGTATCT120800.020.46540637
ACACGTC128300.020.2274913
CAGTCAC126300.020.19168327
AGTCACC127750.020.15614328
TCTCGTA112700.020.07945441
GTATGCC107800.019.82425345
Produced by FastQC (version 0.11.5)