FastQCFastQC Report
Wed 24 Apr 2019
CCRMBACXX_l03_n01_A2S1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCCRMBACXX_l03_n01_A2S1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19859729
Sequences flagged as poor quality0
Sequence length51
%GC52

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACGATCTCGTATGCC267710.13480042955268925TruSeq Adapter, Index 1 (100% over 51bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGTAT46750.029.71622742
ACGTCTG56800.028.3090215
TCGTATG50950.028.28459543
CGTCTGA58400.027.41790616
CGTATGC55350.025.71146244
ATCGGAA67900.023.59182
TCGGAAG69000.023.5079733
CACGTCT71250.022.8202614
CACACGT71750.022.6298912
GTCACAT71400.021.76441229
TCACGAT72050.021.72976335
AGTCACA78000.020.15346328
CAGTCAC80150.020.06178927
ACACGTC81650.020.02374613
TCTCGTA71550.019.82520741
ATCACGA82600.018.92654834
GATCGGA87600.018.4966031
CCAGTCA88000.018.0166326
GTATGCC80700.017.9696545
CATCACG89800.017.78281633
Produced by FastQC (version 0.11.5)