FastQCFastQC Report
Wed 24 Apr 2019
CCRMBACXX_l02_n02_undetermined.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCCRMBACXX_l02_n02_undetermined.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17539628
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTAGATC6150.021.58816532
CACTCTT11150.021.3977932
AGTGTAG6550.019.58297229
GAGTGTA6400.019.33800328
TAGATCT6800.019.19240833
GTGTAGA6800.018.53223830
TCTCGGT8100.016.3876537
TGTAGAT8700.015.5192131
TCCCTAC6450.015.34693059
TCGTGTA9200.014.67246815
GTGTAGG9200.014.672091517
TTCCCTA6750.014.6648048
GATCTCG11000.014.31644535
TGTAGGG9450.014.28361518
ATCGGAA15250.013.8738172
GTAGGGA10100.013.58722719
AGTTAGG1555.797025E-513.069791
TAGGGAA10600.012.94646820
TCGGAAG16250.012.8812763
CCCTACA7700.012.85554710
Produced by FastQC (version 0.11.5)