FastQCFastQC Report
Wed 24 Apr 2019
CCRMBACXX_l02_n02_N2S9.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCCRMBACXX_l02_n02_N2S9.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences26398417
Sequences flagged as poor quality0
Sequence length51
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTAGAAC37900.011.57511311
CGTGACT20800.011.1463062
TCAGGTA48350.010.0038637
GTACTCG46000.09.7368714
CACTCGT41600.09.51791535
AAAATCC87450.09.3946063
TAGAACA48850.09.11887412
TACTCGA48950.08.9658285
GCGGAAA53750.08.9622341
GGTAGAA49600.08.93520510
ATCTAGC44250.08.8971766
ACTAGCG11300.08.76004944
CTCTATA45800.08.74349745
GATCTAG42350.08.7156335
GGAAAAT116400.08.6637521
ACTCGTG45600.08.53527936
CAGGTAG55250.08.4286188
TCACTCG47450.08.39318834
CTCGAAT26450.08.0849781
GCGTGAC32850.08.0173611
Produced by FastQC (version 0.11.5)