FastQCFastQC Report
Wed 24 Apr 2019
CCRMBACXX_l02_n02_N2S8.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCCRMBACXX_l02_n02_N2S8.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences25404884
Sequences flagged as poor quality0
Sequence length51
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTAGAAC32550.011.888111
GTACTCG40250.010.0097084
CACTCGT35750.09.124660535
GAATCGA39250.09.1178364
GGTAGAA42700.09.11460410
TAGAACA43400.09.01989312
TACTCGA46100.08.93454555
AAAATCC79850.08.7384573
TCAGGTA47700.08.7253237
GCGGAAA46050.08.5055551
GGAAAAT109800.08.4465221
TCACTCG38450.08.42643534
CGTGACT19050.08.2711282
AGTACTC53250.08.1157673
AAAAATC78550.08.0236782
CTCTATA42100.08.01579745
CAGGTAG49950.07.92673258
ACTCGTG40150.07.900785436
AAATCCT89500.07.6702774
CGCGATA9500.07.58007329
Produced by FastQC (version 0.11.5)