FastQCFastQC Report
Wed 24 Apr 2019
CCRMBACXX_l02_n02_N2S7.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCCRMBACXX_l02_n02_N2S7.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15024988
Sequences flagged as poor quality0
Sequence length51
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTAGAAC25050.09.5199111
TAGTGCG9000.09.49904913
TCAGGTA28900.08.6408027
CGACGTA14600.08.47721132
TTTCGGC16500.08.45412121
GTACTCG20700.08.3724064
GAATCGA31850.08.338784
CAGGTAG31000.08.1278128
GGAAAAT59150.07.9156711
CGCACTA16550.07.886297732
GCGGTAG16050.07.84960345
TAGTGTA15600.07.789263725
ACGTACA17600.07.54246134
CTTTCGG17100.07.499124520
TAGAGTA16550.07.477414622
TAGAACA31350.07.463367512
AATCGGC11250.07.40236475
TATAAGG16150.07.386265
AATCGTA7353.255991E-107.34928665
AAAAATC51850.07.3365362
Produced by FastQC (version 0.11.5)