FastQCFastQC Report
Wed 24 Apr 2019
CCRMBACXX_l02_n02_N2S6.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCCRMBACXX_l02_n02_N2S6.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences28745143
Sequences flagged as poor quality0
Sequence length51
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTAGAAC43350.012.50713311
TCAGGTA59100.09.7829917
GGTAGAA55900.09.61843810
TAGAACA56600.09.49997812
GTACTCG43150.09.4411664
CAGGTAG60500.09.0357948
CACTCGT43000.08.78943235
GGAAAAT109200.08.5752231
TACTCGA47700.08.4460165
CGTTAAC26350.08.2825221
AATCGAA56150.08.0969025
CTCGAAT25600.08.0015881
TCACTCG46300.07.92107334
AAAATCC82700.07.9200823
ACTCGTG48050.07.912712636
CTCTATA51150.07.87308145
GCGAATT33650.07.822216542
AATTCAC57150.07.7583125
TCCGTTA27850.07.674046519
AGATCAG102150.07.64573054
Produced by FastQC (version 0.11.5)