FastQCFastQC Report
Wed 24 Apr 2019
CCRMBACXX_l02_n02_N2S5.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCCRMBACXX_l02_n02_N2S5.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences26078123
Sequences flagged as poor quality0
Sequence length51
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTAGAAC36950.012.8467911
TCAGGTA49800.010.6612997
CGTGACT20500.010.5410732
GTACTCG49600.010.3009754
TAGAACA46750.010.00961912
CACTCGT44500.09.95929735
TACTCGA52550.09.808075
CTCTATA48350.09.6782545
GGTAGAA49400.09.56336410
AGTACTC57750.09.3543633
GCGGAAA55450.09.2965771
TCACTCG47950.09.24400334
CAGGTAG52850.09.194468
ACTCGTG51100.09.11348436
GAATCGA39550.09.105584
GGAAAAT103450.08.6387151
AAAATCC77300.08.6192043
CGCGAAT33650.08.35708741
CGGCTAT37650.08.07156851
GATCTAG39850.08.0201185
Produced by FastQC (version 0.11.5)