FastQCFastQC Report
Wed 24 Apr 2019
CCRMBACXX_l02_n02_N2S4.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCCRMBACXX_l02_n02_N2S4.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences27497775
Sequences flagged as poor quality0
Sequence length51
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTAGAAC39000.012.63305111
GTACTCG53750.011.1386114
TACTCGA55950.010.57963855
CGTGACT22750.010.38888552
CACTCGT43150.010.21893335
TCAGGTA54800.09.9348297
GGTAGAA50400.09.68612510
TCACTCG45000.09.60003334
ACTCGTG46750.09.287889536
CTCTATA46350.09.27071345
GGAAAAT120800.09.2426131
AAAATCC87450.09.2144623
AGTACTC69950.09.2029093
TAGAACA54750.08.9991312
CAGGTAG58300.08.759468
GCGGAAA56450.08.7329351
AAAAATC77850.08.2403812
AAATCCT94400.08.2018974
CTCGAAT26300.07.95989271
AATTGAC42800.07.83533765
Produced by FastQC (version 0.11.5)