FastQCFastQC Report
Wed 24 Apr 2019
CCRMBACXX_l02_n02_N2S3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCCRMBACXX_l02_n02_N2S3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences30502592
Sequences flagged as poor quality0
Sequence length51
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTAGAAC40750.011.81448511
GTACTCG57000.010.503454
TACTCGA59350.09.9734965
TCAGGTA55900.09.5784347
CGTGACT29150.09.5749582
GCGGAAA65950.09.2155491
GATCTAG63000.09.1813175
TAGAACA52750.09.16965812
ATCTAGC62100.09.0569216
CACTCGT52650.09.01607935
GGTAGAA55550.08.74757410
AGTACTC74650.08.7439643
TCACTCG55900.08.45273834
AAAATCC95250.08.3416113
ACTCGTG56300.08.27195936
CTCTATA57750.08.18088445
AATCGAA56800.08.1626525
GGAAAAT135050.08.0338681
ACTAGCG18100.07.954983744
AATTGAC49800.07.90899135
Produced by FastQC (version 0.11.5)