FastQCFastQC Report
Wed 24 Apr 2019
CCRMBACXX_l02_n02_N2S2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCCRMBACXX_l02_n02_N2S2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences31197104
Sequences flagged as poor quality0
Sequence length51
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTAGAAC39600.011.1350211
CGTGACT25750.010.4896222
CACTCGT49750.010.08400235
TCACTCG52950.09.56085734
ACTCGTG53000.09.42345236
CTCTATA54500.09.20538645
GTACTCG51600.09.1601024
GGAAAAT146350.08.7979771
GCGGAAA68350.08.4969031
TACTCGA55150.08.4885995
TCAGGTA57300.08.2450667
GGTAGAA54600.08.24052610
AAAATCC100800.08.195053
ATCTAGC47200.08.1029416
GCGTGAC38850.07.9959041
TAGAACA53000.07.98031112
CTCGAAT29950.07.89172551
GAATTGA82500.07.7208164
AGTACTC69300.07.5677853
CTCGTGA72300.07.53051437
Produced by FastQC (version 0.11.5)