FastQCFastQC Report
Wed 24 Apr 2019
CCRMBACXX_l02_n02_N2S1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCCRMBACXX_l02_n02_N2S1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences30355214
Sequences flagged as poor quality0
Sequence length51
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CACTCGT59550.011.37127435
CGTGACT28950.010.807452
CTCTATA61850.010.69402845
GTAGAAC37700.010.68171811
TCACTCG60900.010.67747734
ACTCGTG62700.010.65671436
GTACTCG52000.010.5180074
GCGGAAA74200.010.13242151
TACTCGA55550.09.60244755
GGAAAAT132000.09.4131681
AGTACTC67650.09.1497923
GCGTGAC38150.09.0865041
AAACTAA88150.08.5278945
TCGTGAG84200.08.47031338
GAATCGA45000.08.40286354
CTCGTGA81450.08.39717937
GCTAGTT87150.08.36407212
AAAAATC88400.08.3517712
AAAATCC93500.08.3293443
GAAACTA95750.08.3213744
Produced by FastQC (version 0.11.5)