FastQCFastQC Report
Wed 24 Apr 2019
CCRMBACXX_l02_n01_undetermined.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCCRMBACXX_l02_n01_undetermined.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17539628
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGTCTG13550.034.2885715
CGTCTGA13850.033.54585616
CACGTCT15250.030.60406914
CACACGT15650.030.10783612
CAGTCAC11650.027.87519827
ACACGTC17550.027.10270513
TCGGAAG22700.024.8929673
CCAGTCA14750.023.6982426
AGCACAC20350.023.48338910
GCACACG20800.023.4047811
ATCGGAA24200.023.3578722
GATCGGA25500.022.1166821
CGGAAGA27050.021.0549814
ACCGATC6300.019.10349534
GAGACCG7050.018.93365131
AACTCCA24550.018.54527522
CCGAGAC7250.018.35932529
CTCCAGT22400.018.04882824
AGACCGA7750.017.54442432
AGAGCAC29800.016.0364768
Produced by FastQC (version 0.11.5)