FastQCFastQC Report
Wed 24 Apr 2019
CCRMBACXX_l02_n01_N2S9.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCCRMBACXX_l02_n01_N2S9.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences26398417
Sequences flagged as poor quality0
Sequence length51
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCAGATCTCGTATGCC345540.1308942123309894TruSeq Adapter, Index 9 (100% over 51bp)
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT269440.10206672619801407No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGTAT61400.028.42750542
AGTCACG69050.028.27708828
TCGTATG64000.027.37961243
CGTCTGA76300.026.53362816
ACGTCTG77000.026.32162715
CGTATGC71450.024.30411344
GTCACGA85850.022.8745529
TCGGAAG93100.022.3779223
ATCGGAA93500.022.091152
CACGTCT94950.021.58251614
CACACGT97500.021.06419212
CAGTCAC95850.020.98089827
TCACGAT98250.019.9646930
TCTCGTA91850.018.97772641
ACACGTC111900.018.57464613
GATCGGA121000.017.2576621
CCAGTCA115800.017.23033526
ACGATCA116200.017.15185732
GTATGCC107000.016.71341545
GAGCACA131900.016.26989
Produced by FastQC (version 0.11.5)