FastQCFastQC Report
Wed 24 Apr 2019
CCRMBACXX_l02_n01_N2S8.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCCRMBACXX_l02_n01_N2S8.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences25404884
Sequences flagged as poor quality0
Sequence length51
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT292270.11504480791961105No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGTCTG67500.025.66082215
CGTCTGA70750.024.70462216
GTCACAC76900.022.08534629
CTCGTAT39200.021.19893342
TCGGAAG86050.020.551493
ATCGGAA86250.020.4267442
CACGTCT87300.020.27888714
CACACGT87650.020.09525112
CAGTCAC90900.019.15405527
TCGTATG41450.018.58210843
AGTCACA93800.018.20214528
CACACTT97200.017.79694431
ACACGTC101350.017.66741213
TCACACT105850.016.3000230
GATCGGA112950.015.8181751
CGTATGC44350.015.64049444
AATCTCG77300.015.49388639
ACACTTG111650.015.45347832
CCAGTCA110500.015.41051926
CGGAAGA119750.015.2939814
Produced by FastQC (version 0.11.5)