FastQCFastQC Report
Wed 24 Apr 2019
CCRMBACXX_l02_n01_N2S7.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCCRMBACXX_l02_n01_N2S7.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15024988
Sequences flagged as poor quality0
Sequence length51
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCTCGTATGCC442870.294755643066071TruSeq Adapter, Index 7 (100% over 51bp)
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT167260.11132122035638231No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGTAT66550.033.60384842
ACGTCTG95900.032.3698515
CGTCTGA97750.031.73421116
TCGTATG67500.031.36446843
CACACGT104800.029.85699512
ATCGGAA107750.029.2135752
CACGTCT107900.028.89495314
TCGGAAG110450.028.5391333
CAGTCAC107450.028.22043627
CGTATGC74000.027.60510444
ACACGTC115750.027.32401513
TCTCGTA84200.027.012841
AGTCACC113300.026.82289728
GATCGGA121000.026.2207741
CGGAAGA125400.025.4417554
CCAGTCA119800.025.34878526
TCCAGTC121900.025.05972525
GCACACG127100.024.760111
ACTCCAG127950.024.49102423
GTATGCC84300.024.44605845
Produced by FastQC (version 0.11.5)