FastQCFastQC Report
Wed 24 Apr 2019
CCRMBACXX_l02_n01_N2S6.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCCRMBACXX_l02_n01_N2S6.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences28745143
Sequences flagged as poor quality0
Sequence length51
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT331410.11529252089648676No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGTAT48650.021.43374342
AGTCACG55300.021.27439128
TCGTATG51250.019.77595343
ACGTCTG61300.019.55902915
CGTCTGA66350.018.2737816
TATCTCG61950.018.13431239
GTCACGC67100.017.56666629
CGTATGC62900.016.0773544
ATCGGAA81750.015.1382232
TCGGAAG83800.014.874473
CACGTCT83450.014.63706814
CACACGT85250.014.56549612
CAGTCAC86800.014.25356727
CGCCAAT81350.014.15785733
ACGCCAA83450.013.74766632
CACGCCA86250.013.56203431
TCACGCC93700.012.91585830
ACACGTC100500.012.73581713
GCCAATA95700.012.481894534
TATAGGC65800.011.96527627
Produced by FastQC (version 0.11.5)