FastQCFastQC Report
Wed 24 Apr 2019
CCRMBACXX_l02_n01_N2S5.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCCRMBACXX_l02_n01_N2S5.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences26078123
Sequences flagged as poor quality0
Sequence length51
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATCTCGTATGCC2559780.9815813814514182TruSeq Adapter, Index 5 (100% over 51bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGTCTG329950.041.2735815
CGTCTGA330000.041.0764616
TCGTATG314350.040.61711543
CTCGTAT309500.040.517342
ATCGGAA347450.039.7497142
TCGGAAG351050.039.3135073
CGTATGC324900.039.02092744
CACGTCT351100.038.9154314
CACACGT353000.038.75694712
GTCACAC341100.038.63844329
CAGTCAC351100.037.64045727
ACACGTC366200.037.45820213
AGTCACA354600.037.10428
GATCGGA374250.036.9601861
AGTGATC343150.036.80467236
TCTCGTA340700.036.77803841
CACAGTG353850.036.7700733
ACACAGT354850.036.7234332
GTATGCC352050.036.1076145
CGGAAGA385650.035.7980164
Produced by FastQC (version 0.11.5)