FastQCFastQC Report
Wed 24 Apr 2019
CCRMBACXX_l02_n01_N2S4.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCCRMBACXX_l02_n01_N2S4.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences27497775
Sequences flagged as poor quality0
Sequence length51
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGTCTG47250.021.5664715
CGTCTGA50000.020.5152816
CTCGTAT37050.017.627842
TCGTATG37650.017.22790343
AGTCACT58800.016.67983828
ATCGGAA64900.015.8095262
TCGGAAG65800.015.7290413
CACGTCT69100.015.0725114
CACACGT70950.014.77461412
ACTGACC71100.013.79447232
CAGTCAC74000.013.64888827
CGTATGC47050.013.35517344
ACACGTC86000.013.156857513
GTCACTG80350.012.34625729
AATCTCG74350.011.71841639
CACTGAC85650.011.58227431
TATAGGC55900.011.50898127
GATCGGA91800.011.4962591
ATCGGTG95100.010.9755746
TGACCAA93250.010.63876834
Produced by FastQC (version 0.11.5)