FastQCFastQC Report
Wed 24 Apr 2019
CCRMBACXX_l02_n01_N2S3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCCRMBACXX_l02_n01_N2S3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences30502592
Sequences flagged as poor quality0
Sequence length51
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACTTAGG51150.024.97952732
ACGTCTG56850.023.06813615
CGTCTGA58750.022.39867816
CTCGTAT51200.020.7634742
TCACTTA62850.020.40064830
TTAGGCA62350.020.3846634
TCGTATG51250.019.9530143
CTTAGGC65250.019.58168633
GTCACTT67300.019.01829529
AGTCACT67800.019.01075428
CGTATGC54250.018.64202344
CACTTAG69500.018.3191831
CACGTCT78300.017.06471814
ATCGGAA77750.016.9583972
TCGGAAG79250.016.9487253
CACACGT86500.015.49903112
CAGTCAC85650.015.39021327
TAGGCAT88500.014.5926535
ACACGTC98550.013.85499813
GATCGGA108550.012.2511231
Produced by FastQC (version 0.11.5)