FastQCFastQC Report
Wed 24 Apr 2019
CCRMBACXX_l02_n01_N2S2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCCRMBACXX_l02_n01_N2S2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences31197104
Sequences flagged as poor quality0
Sequence length51
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGTCTG67450.024.8460715
CTCGTAT55300.024.39411742
CGTCTGA70200.023.96888716
TCGTATG56500.022.96030643
CGTATGC63700.020.1529744
TATCTCG76600.020.10242539
CACCGAT83200.019.5748931
ACCGATG85100.019.29663532
TCGGAAG90300.019.1610263
ATCGGAA89050.019.0771452
CACGTCT90000.019.02065714
GATGTAT86000.018.91480435
CACACGT93000.018.45546312
CAGTCAC100900.017.18884327
ACACGTC108400.016.1655913
AGTCACC109400.015.85332928
GTATCTC108000.015.27447138
TCTCGTA94100.014.78943641
TGTATCT112600.014.76757437
GATCGGA120250.014.2964481
Produced by FastQC (version 0.11.5)