FastQCFastQC Report
Wed 24 Apr 2019
CCRMBACXX_l02_n01_N2S1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCCRMBACXX_l02_n01_N2S1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences30355214
Sequences flagged as poor quality0
Sequence length51
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGTCTG53050.023.27913715
CGTCTGA55500.022.37309316
CTCGTAT48000.021.2075442
TCGTATG51150.020.51883743
TCGGAAG70850.018.037653
CGTATGC58100.017.71546444
ATCGGAA71250.017.6534332
CACGTCT74750.017.00267214
CACACGT82350.015.57009212
CAGTCAC84250.015.21895627
TCACGAT84700.014.3709235
ACACGTC95000.013.89934613
GTCACAT89950.013.70437729
AGTCACA91300.013.69884328
ATCACGA96650.012.63865334
TCTCGTA84950.012.32704741
CCAGTCA105350.012.085422526
GATCGGA105950.011.999941
AGCACAC115000.011.95152410
CGGAAGA113800.011.7835324
Produced by FastQC (version 0.11.5)