FastQCFastQC Report
Wed 24 Apr 2019
CCRMBACXX_l01_n02_undetermined.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCCRMBACXX_l01_n02_undetermined.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17082576
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CACTCTT10100.018.9367392
GTAGATC5900.016.01923632
GTGTAGA5500.015.549314530
GAGTGTA5400.015.4208128
AGTGTAG5550.015.00372429
TCTCGGT6300.014.99779137
TAGATCT6400.014.76708433
TTCCCTA6950.012.9490238
ATCGGAA13850.012.50974752
TCGTGTA7750.012.481667515
GACTTAA1506.456446E-412.0001315
GATCTCG10000.011.92268635
GTGTAGG8150.011.86931317
CTCTATA6300.011.78404845
TGTAGGG8250.011.72616518
CACTCGT5950.011.72041435
TCCCTAC6600.011.5901189
TATAGTC2351.7635757E-611.48730116
TCGGAAG15100.011.4739713
AGCGGTT7150.011.32789910
Produced by FastQC (version 0.11.5)