FastQCFastQC Report
Wed 24 Apr 2019
CCRMBACXX_l01_n02_P2S9.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCCRMBACXX_l01_n02_P2S9.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences45412426
Sequences flagged as poor quality0
Sequence length51
%GC55

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTGACT48500.014.9854382
GCGTGAC63450.012.6967521
GTACTCG102650.012.2093224
TACTCGA103850.012.0249085
AGTACTC117950.011.179043
GTAGAAC54100.011.144855511
GTGACTC74800.010.3481253
GGAAAAT180500.09.54976751
CACTCGT100600.09.45856535
AAAATCC140600.09.3141363
CTCTATA105500.09.29721245
CGCGAAT53850.09.23292241
GCGGAAA119100.09.220391
ACTCGTG105250.09.12635236
TCACTCG108250.08.89533534
CTCGAAT47450.08.7735781
CTAGCAC89850.08.66284414
TCAGGTA77750.08.5948337
GCTCTAG95600.08.54251211
TAGAACA70950.08.33914712
Produced by FastQC (version 0.11.5)