FastQCFastQC Report
Wed 24 Apr 2019
CCRMBACXX_l01_n02_P2S8.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCCRMBACXX_l01_n02_P2S8.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences59327627
Sequences flagged as poor quality0
Sequence length51
%GC54

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTGACT46900.011.2266062
GTAGAAC76400.011.16038511
GTACTCG105950.010.597214
TACTCGA113300.09.7905845
CACTCGT100350.09.59434735
AGTACTC126150.09.3107363
GAATCGA96600.09.293684
CTCGAAT61500.09.0743781
GCGTGAC67600.09.0211721
GCGGAAA123050.08.997541
AATCGAA112050.08.935935
TCAGGTA103650.08.8348857
GGAAAAT250850.08.8181951
ACTCGTG109600.08.80525736
AAAATCC184500.08.6467053
TAGAACA105850.08.26755412
GGTAGAA102150.08.23711210
CTCTATA118550.08.19788145
TCACTCG115300.08.17581834
CGTTAAC45500.08.15916121
Produced by FastQC (version 0.11.5)