FastQCFastQC Report
Wed 24 Apr 2019
CCRMBACXX_l01_n02_P2S7.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCCRMBACXX_l01_n02_P2S7.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9785798
Sequences flagged as poor quality0
Sequence length51
%GC54

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTAGAAC16900.013.71130911
GTACTCG23650.011.2266544
TCAGGTA21250.011.2236817
AGTACTC27100.010.6280483
TACTCGA24800.010.5246055
TAGAACA21950.010.45379812
GGTAGAA22700.010.20829310
CAGGTAG22950.010.0974028
GAATCGA19150.09.0473514
CTCGAAT12100.08.9270191
AGGTAGA26150.08.86154659
GAATTGA23900.08.7555724
AGATCAG38750.08.5358184
ACTCGAG32150.08.468456
ACGCGGG12850.08.40383734
AAAATCC31100.08.1758123
TGCGCAC10600.08.0653229
CTCGGAT16700.07.95034741
AATTGAC17200.07.84914355
AGAGATC41450.07.38294122
Produced by FastQC (version 0.11.5)