FastQCFastQC Report
Wed 24 Apr 2019
CCRMBACXX_l01_n02_P2S6.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCCRMBACXX_l01_n02_P2S6.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences25741090
Sequences flagged as poor quality0
Sequence length51
%GC54

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTAGAAC37200.012.15599511
CGTGACT23000.011.3483252
CACTCGT50950.09.66909635
TCAGGTA53050.09.5850847
GTACTCG43950.09.5734644
GCGTGAC30300.09.357611
CTCGAAT28150.09.3528611
GGTAGAA50100.09.20575610
CGCGAAT34550.09.18130141
TAGAACA51000.09.17516112
TACTCGA46900.08.8753455
GCGAATT36600.08.851521542
CTCTATA56900.08.65864345
GCGGAAA63400.08.62491
ACTCGTG57600.08.59200636
TCACTCG58000.08.57260234
GGAAAAT101200.08.4274411
CAGGTAG54500.08.4214228
CGGCTAT42250.08.3619971
AGTACTC56300.08.2329313
Produced by FastQC (version 0.11.5)