FastQCFastQC Report
Wed 24 Apr 2019
CCRMBACXX_l01_n02_P2S5.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCCRMBACXX_l01_n02_P2S5.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22585236
Sequences flagged as poor quality0
Sequence length51
%GC54

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTGACT17800.011.7562782
GTAGAAC29150.011.42238911
GTACTCG49000.011.066584
TACTCGA51100.010.5677575
AGTACTC55800.010.2423973
AAAATCC72850.09.5440143
GCGTGAC25350.09.3207731
AAAAATC67150.09.2149632
CTCGAAT24300.09.1678931
GAATCGA41600.08.8163084
AATCGAA47200.08.7237175
CACTCGT37750.08.64043935
TCAGGTA42200.08.6372767
TAGAACA38850.08.57018212
GGAAAAT92350.08.3335461
GCGGAAA48150.08.2253991
CGCGAAT27400.08.12875741
GGTAGAA42100.08.1227310
CAGGTAG41850.08.1177298
TCACTCG40950.08.07621434
Produced by FastQC (version 0.11.5)