FastQCFastQC Report
Wed 24 Apr 2019
CCRMBACXX_l01_n02_P2S4.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCCRMBACXX_l01_n02_P2S4.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences23522706
Sequences flagged as poor quality0
Sequence length51
%GC54

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTGACT26550.011.7804172
GTACTCG57750.010.8315894
GCGTGAC31500.010.7157951
TACTCGA58500.010.6157725
AGTACTC65800.09.6092183
GTAGAAC29300.09.36750911
AAAATCC78950.09.09172253
GGAAAAT100450.08.8041271
CTAGCAC43100.08.24675514
GCTCTAG47000.08.18521611
AATCGGA21200.08.0663335
AAAAATC69800.08.0592572
CTCTATA49900.08.02489645
AATTGAC38300.07.98984865
CACTCGT50600.07.82440935
GTGACTC43150.07.76985843
TCACTCG50100.07.679039534
ACTCGAG87650.07.65000726
GCGGAAA57800.07.59191
CGGCTAT32350.07.5822121
Produced by FastQC (version 0.11.5)