FastQCFastQC Report
Wed 24 Apr 2019
CCRMBACXX_l01_n02_P2S3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCCRMBACXX_l01_n02_P2S3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences24491133
Sequences flagged as poor quality0
Sequence length51
%GC55

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACTCG58900.013.3321544
TACTCGA60500.012.8307795
GTAGAAC30650.012.69850411
CGTGACT22400.012.656992
AGTACTC64350.012.3082623
CTCGAAT23750.011.9384931
GAATCGA38100.010.7482214
GCGTGAC32200.010.3430441
GGTAGAA41500.09.70393910
TAGAACA42050.09.09496912
TCAGGTA46050.09.0878497
AATCGAA46700.08.9615965
ACTCGAG94300.08.9476476
AAAATCC72550.08.9011713
GTGACTC34850.08.6517643
GGAAAAT96350.08.4780791
CAGGTAG49700.08.2389768
ACATGCG25750.08.213726
CACTCGT42200.08.209135
CGGGAAT13450.08.0308491
Produced by FastQC (version 0.11.5)