FastQCFastQC Report
Wed 24 Apr 2019
CCRMBACXX_l01_n02_P2S2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCCRMBACXX_l01_n02_P2S2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences23535767
Sequences flagged as poor quality0
Sequence length51
%GC54

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTGACT23150.014.6768082
GTACTCG49600.012.4751184
GCGTGAC31400.011.8965491
TACTCGA53100.011.6104685
AGTACTC58850.011.2409223
GTAGAAC27600.010.67814611
CACTCGT55200.09.73963435
CTCTATA58200.09.62489945
GTGACTC38900.09.5441093
CTCGAAT23600.09.5352171
GCGGAAA65350.09.4695581
ACTCGTG58550.09.29778936
GGAAAAT106450.09.2591491
TCACTCG59350.09.24942634
AAAATCC78200.09.0349123
GCTCTAG46600.08.69000911
AAATCCT86500.08.6620724
AATCGGA19850.08.38812355
GAATCGA35800.08.2963034
TAGAACA37550.08.207812
Produced by FastQC (version 0.11.5)