FastQCFastQC Report
Wed 24 Apr 2019
CCRMBACXX_l01_n02_P2S1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCCRMBACXX_l01_n02_P2S1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences23137863
Sequences flagged as poor quality0
Sequence length51
%GC54

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTGACT22850.014.5741142
GCGTGAC30250.012.2744461
GTACTCG46950.011.501844
GTAGAAC27300.011.20745211
TACTCGA47650.011.0495515
GTGACTC34900.010.18676953
AGTACTC58700.09.8131093
TTACGCG6300.09.2850478
AATCGAA45500.09.000195
GAATCGA36200.08.8883094
GGAAAAT103150.08.81364251
TAGAACA34800.08.72708412
AAAATCC73700.08.6096683
TCAGGTA42300.08.2977427
CTAGCAC43200.08.2797414
CTCGAAT21750.08.0700951
GCTCTAG45900.07.989240611
CACTCGT48250.07.97234735
GGTAGAA39500.07.859972510
CGCGAAT25200.07.85623841
Produced by FastQC (version 0.11.5)