FastQCFastQC Report
Wed 24 Apr 2019
CCRMBACXX_l01_n01_undetermined.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCCRMBACXX_l01_n01_undetermined.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17082576
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCTGA12100.032.0314316
ACGTCTG12300.031.32845115
CACACGT14150.027.39083312
CACGTCT14850.025.94881814
ACACGTC15500.025.1497213
TCGGAAG20000.024.086993
ATCGGAA20350.023.6954692
CAGTCAC11900.020.99192827
GATCGGA23150.020.8211361
AGCACAC19200.020.41984210
CCAGTCA13600.019.68822126
CGGAAGA24750.019.4642354
GCACACG20400.019.32849711
AACTCCA21650.017.08375222
CTCCAGT21050.016.09422524
AGTCACT5750.014.87426428
CCGAGAC6600.014.32843729
AGACCGA6450.013.6411632
GAACTCC29500.013.21847621
ACCGATC6500.012.88596934
Produced by FastQC (version 0.11.5)