FastQCFastQC Report
Wed 24 Apr 2019
CCRMBACXX_l01_n01_P2S9.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCCRMBACXX_l01_n01_P2S9.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences45412426
Sequences flagged as poor quality0
Sequence length51
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCAGATCTCGTATGCC535120.11783558975686523TruSeq Adapter, Index 9 (100% over 51bp)
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT487340.10731424037993477No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGTCTG117000.030.01385315
AGTCACG115750.029.71605928
CGTCTGA121800.028.68328716
CTCGTAT98500.027.22634142
TCGTATG100150.026.57604443
TCGGAAG149500.024.1993
ATCGGAA149000.024.1905122
GTCACGA143700.024.06145129
CACGTCT152600.023.29202714
CGTATGC113600.022.71605744
CACACGT158650.022.64485512
TCACGAT158800.021.83015830
CAGTCAC162100.021.55227727
ACACGTC184750.019.7988913
GATCGGA196550.018.5353781
TCTCGTA152750.017.96873941
AGCACAC207450.017.92520510
ACGATCA194800.017.88822432
CCAGTCA197150.017.74346226
CGGAAGA209350.017.5065544
Produced by FastQC (version 0.11.5)