FastQCFastQC Report
Wed 24 Apr 2019
CCRMBACXX_l01_n01_P2S8.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCCRMBACXX_l01_n01_P2S8.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences59327627
Sequences flagged as poor quality0
Sequence length51
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT682120.11497510257742148No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGTCTG128100.025.09515215
CGTCTGA136500.023.583816
GTCACAC157650.019.94894629
TCGGAAG166150.019.702423
ATCGGAA166400.019.6601092
CACGTCT172900.019.08718514
CACACGT173900.018.82219112
CAGTCAC184650.017.53146727
CTCGTAT70800.016.88762942
CACACTT192000.016.75488931
AGTCACA194200.016.48399428
ACACGTC214650.015.85677213
CCAGTCA219550.014.67291326
TCGTATG76350.014.56926943
GATCGGA229650.014.4529851
TCACACT228850.014.16510730
ACACTTG229600.014.128633532
TCCAGTC231550.014.09708825
TTGAATC231500.013.55777936
CGGAAGA248850.013.45309454
Produced by FastQC (version 0.11.5)